GSE114397

syn77106056

Created By Aditya Nath aditya.nath

doi: 10.1016/j.cell.2018.05.061
title: Single cell RNA-seq and ATAC-seq of EMT induced by TGFbeta stimulation and Zeb1 overexpression
funder: NIH-NCI
series:
creator: van Dijk David
license: CC-BY 4.0
species: Homo sapiens
studyId: syn7315805
subject: HMLE
ageGroup:
citation: van Dijk D et al. (2018). Recovering Gene Interactions from Single-Cell Data Using Data Diffusion. Cell. 174(3):716-729.e27. doi: 10.1016/j.cell.2018.05.061
dataType: chromatin activity gene expression
keywords: HMLE breast cancer cell line
accessType: Open Access
contributor:
description: We report the application of single cell RNA-sequencing using indrop on an HMLE breast cancer cell line that we induced to undergo EMT. We measured 7523 single cells after 8 and 10 days of stimulation with TGFbeta. In addition, we measured 3496 single cells in an engineered HMLE cell line with Dox inducible Zeb1, after 2 days of stimulation with Doxycycline. Finally, we performed ATAC-seq on CD44 sorted HMLE cells after 8 days of stimulation with TGFbeta.
grantNumber: CA209975
diseaseFocus:
downloadType: Synapse Hosted
alternateName: GSE114397
manifestation:
specimenCount: 4
yearProcessed: 2018
countryOfOrigin:
individualCount: 4
visualizeDataOn:
dataUseModifiers:
conditionsOfAccess: No restriction
measurementTechnique: ATAC-seq RNA-seq single-cell RNA-seq
externalRepositoryUri: geo:GSE114397
includedInDataCatalog:

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