GSE115870

syn77105451

Created By Aditya Nath aditya.nath

doi: 10.1016/j.celrep.2018.08.005
title: Real-time quantitative PCR analysis of induced pluripotent derived endothelial cells
funder: NIH-NCI
series:
creator: Gerecht Sharon Smith Quinton
license: CC-BY 4.0
species: Homo sapiens
studyId: syn7349766
subject:
ageGroup:
citation: Smith Q et al. (2018). Differential HDAC6 Activity Modulates Ciliogenesis and Subsequent Mechanosensing of Endothelial Cells Derived from Pluripotent Stem Cells. Cell Rep. 24(7):1930. doi: 10.1016/j.celrep.2018.08.005
dataType: gene expression
keywords:
accessType: Open Access
contributor:
description: CD31+/VECad+ hiPSC-ECs were purified through magnetic activated cell sorting. Expanded endothelial cells were then subjected to serum starvation conditions for 24 hours by culturing in DMEM. After serum starvation, total RNA was extracted. We used a custom Thermo Fisher Scientific PCR assay panel to quantify gene expression relevant to ciliogenesis, cytoskeletal integrity, Wnt signaling, pluripotency and endothelial phenotype.
grantNumber: CA210173
diseaseFocus:
downloadType: Synapse Hosted
alternateName: GSE115870
manifestation:
specimenCount: 3
yearProcessed: 2018
countryOfOrigin:
individualCount: 1
visualizeDataOn:
dataUseModifiers:
conditionsOfAccess: No restriction
measurementTechnique: flow cytometry quantitative PCR
externalRepositoryUri: geo:GSE115870
includedInDataCatalog:

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