GSE120261

syn77096468

Created By Aditya Nath aditya.nath

doi: 10.1038/s41598-019-41235-9
title: In situ 10-cell RNA sequencing in tissue and tumor biopsy samples
funder: NIH-NCI
series:
creator: Janes Kevin Singh Shambhavi
license: CC-BY 4.0
species: Homo sapiens Mus musculus
studyId: syn17084062
subject:
ageGroup:
citation: Singh S et al. (2019). In situ 10-cell RNA sequencing in tissue and tumor biopsy samples. Sci Rep. 9(1):4836. doi: 10.1038/s41598-019-41235-9
dataType: gene expression
keywords:
accessType: Open Access
contributor:
description: We combined the tissue preservation and single-cell resolution of laser capture with an improved preamplification procedure enabling RNA sequencing of 10 microdissected cells. This in situ 10-cell RNA sequencing (10cRNA-seq) can exploit fluorescent reporters of cell type in genetically engineered mice and is compatible with freshly cryoembedded clinical biopsies from patients. By using small pools of microdissected cells, 10cRNA-seq thus results in improved per-cell reliability and sensiti...
grantNumber: CA215794
diseaseFocus:
downloadType: Synapse Hosted
alternateName: GSE120261
manifestation:
specimenCount: 168
yearProcessed: 2018
countryOfOrigin:
individualCount: 168
visualizeDataOn:
dataUseModifiers:
conditionsOfAccess: No restriction
measurementTechnique: RNA-seq single-cell RNA-seq
externalRepositoryUri: geo:GSE120261
includedInDataCatalog:

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