GSE129578

syn77092959

Created By Aditya Nath aditya.nath

doi: 10.1038/s41592-019-0433-8
title: MULTI-seq: Universal sample multiplexing for single-cell RNA sequencing using lipid-tagged indices
funder: NIH-NCI
series:
creator: McGinnis Christopher Gartner Zev
license: CC-BY 4.0
species: Homo sapiens Mus musculus
studyId: syn9774783
subject:
ageGroup:
citation: McGinnis CS et al. (2019). MULTI-seq: sample multiplexing for single-cell RNA sequencing using lipid-tagged indices. Nat Methods. 16(7):619-626. doi: 10.1038/s41592-019-0433-8
dataType: gene expression
keywords:
accessType: Open Access
contributor:
description: We report the development of MULTI-seq: a scRNA-seq and snRNA-seq sample multiplexing approach using lipid- or cholesterol-modified oligonucleotides. We demonstrate MULTI-seq utility in the following scRNA-seq and snRNA-seq contexts: (1) Live HEKs and HMECs with and without stimulation with TGF-β, (2) Purified nuclei from HEKs, MEFs, and Jurkats stimulated for 0-24 hrs with ionomycin and PMA, (3) 96 unique HMEC cultures (with one technical replicate), and (4) human metastases and mouse immune...
grantNumber: CA199315
diseaseFocus:
downloadType: Synapse Hosted
alternateName: GSE129578
manifestation:
specimenCount: 24
yearProcessed: 2019
countryOfOrigin:
individualCount: 24
visualizeDataOn:
dataUseModifiers:
conditionsOfAccess: No restriction
measurementTechnique: RNA-seq single-cell RNA-seq
externalRepositoryUri: geo:GSE129578
includedInDataCatalog:

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