GSE111653

syn77091669

Created By Aditya Nath aditya.nath

doi: 10.1158/1541-7786.MCR-18-0345
title: A consensus hypoxia signature in breast cancer
funder: NIH-NCI
series:
creator: Fertig Elana Gilkes Daniele
license: CC-BY 4.0
species: Homo sapiens
studyId: syn7349766
subject:
ageGroup:
citation: Ye IC et al. (2018). Molecular Portrait of Hypoxia in Breast Cancer: A Prognostic Signature and Novel HIF-Regulated Genes. Mol Cancer Res. 16(12):1889-1901. doi: 10.1158/1541-7786.MCR-18-0345
dataType: gene expression
keywords:
accessType: Open Access
contributor:
description: We exposed a panel of 32 breast cancer cell lines or normal human mammary epithelial cells to 20% or 1% O2 concentration for 24h. Total RNA was extracted from cells using TRIzol (Invitrogen) and treated with DNase I (Ambion). All samples had a RIN value of >9.0 when measured on an Agilent Bioanalyzer. Libraries for RNA-Seq were prepared with KAPA Stranded RNA-Seq Kit. The workflow consisted of mRNA enrichment, cDNA generation, end repair to generate blunt ends, A-tailing, adaptor ligation and...
grantNumber: CA210173
diseaseFocus:
downloadType: Synapse Hosted
alternateName: GSE111653
manifestation:
specimenCount: 64
yearProcessed: 2018
countryOfOrigin:
individualCount: 64
visualizeDataOn:
dataUseModifiers:
conditionsOfAccess: No restriction
measurementTechnique: flow cytometry immunohistochemistry RNA-seq quantitative PCR
externalRepositoryUri: geo:GSE111653
includedInDataCatalog:

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