GSE136907

syn77068405

Created By Aditya Nath aditya.nath

doi: 10.1161/CIRCULATIONAHA.119.041231
title: Individual nucleotide resolution Crosslinking Immunoprecipitation (iCLIP)-seq
funder: NIH-NCI
series:
authors: Chen Zhen Qi Zhijie Zhong Sheng
creator: Chen Zhen Qi Zhijie Zhong Sheng
license: CC-BY 4.0
species: Homo sapiens
studyId: syn22115279
subject:
ageGroup:
citation: Tang X et al. (2020). Suppression of Endothelial AGO1 Promotes Adipose Tissue Browning and Improves Metabolic Dysfunction. Circulation. 142(4):365-379. doi: 10.1161/CIRCULATIONAHA.119.041231
dataType: gene expression
keywords:
accessType: Open Access
contributor:
description: Purpose: to identify the AGO1-bound miRNAs and mRNAs in human endothelial cells subject to hypoxic condition (2% oxygen) Methods: human microvascular endothelial cells was cultured under normoxia (20% oxygen) or hypoxia (2%) oxygen for 24 hours. Total RNA was extracted and RNA and small RNA libraries were prepared for Seq. Results: We found substantial changes in AGO1-bound mRNAs due to hypoxia. A portion of CLIP-seq reads also reveal direct binding to miRNA to mRNA. Conclusions: hypoxia indu...
grantNumber: CA250046
diseaseFocus:
downloadType: Synapse Hosted
alternateName: GSE136907
manifestation:
specimenCount: 4
yearProcessed: 2019
countryOfOrigin:
individualCount: 4
visualizeDataOn:
dataUseModifiers:
conditionsOfAccess: No restriction
measurementTechnique: RNA-seq
externalRepositoryUri: geo:GSE136907
includedInDataCatalog:

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