We are preparing predictions for the CAGI challenge on your TSC2 DMS data, and I’d appreciate a quick clarification on the challenge description: “Two diverse libraries of plasmids encoding TSC2 (tuberin) or RapGAP domain missense variants were generated using site-saturation mutagenesis......” Am I correct that Fowler’s lab generated two separate libraries, one covering the TSC2 tuberin domain and another limited to the RapGAP domain? Were the effects of variants in the libraries assessed in the two domain constructs or in the full-length protein? Could we have access to the exact sequence of the domain used in the construct? Apologies if I’m missing something obvious, and many thanks in advance for the clarification. 3BIOTeam Bruxelles