Hello, I am analyzing RNA-seq data (syn22024496) for ROSMAP blood samples and have question about fastq files downloaded from synapse.org. There are 3 batches: batch1 and batch2 are ok, but there are 6 samples in batch3 which fastq files have two end reads with different Sequencer/run/flowcell/lane. Usually the paired reads for each sample are the same Sequencer/run/flowcell/lane. Is there any reason or problem with batch3 fastq files? Thanks, Chunling The fastq headers are extracted as below: Sample_001 @A00132:144:HK735DMXX:2:1101:2311:1016 1:N:0:TAGCATAACC+NCTATGCGCA Sample_001 @A00404:186:HN3M2DMXX:1:1101:9607:1000 2:N:0:TAGCATAACC+GCTATGCGCA Sample_227 @A00132:144:HK735DMXX:1:1101:2935:1000 2:N:0:TTCACGAGAC+NGATTGTTAC Sample_227 @A00404:186:HN3M2DMXX:1:1101:2790:1000 1:N:0:TTCACGAGAC+AGATTGTTAC Sample_328 @A00132:144:HK735DMXX:1:1101:2248:1000 1:N:0:TGGATCTGGC+NAATGCTGAA Sample_328 @A00404:186:HN3M2DMXX:1:1101:3423:1000 2:N:0:TGGATCTGGC+CAATGCTGAA Sample_436 @A00132:144:HK735DMXX:1:1101:1940:1000 1:N:0:AAGCATCTTG+NATTCAACAA Sample_436 @A00404:186:HN3M2DMXX:1:1101:3857:1000 2:N:0:AAGCATCTTG+CATTCAACAA Sample_499 @A00132:144:HK735DMXX:1:1101:9019:1016 1:N:0:TATCACTCTG+NACGTTACAT Sample_499 @A00404:186:HN3M2DMXX:1:1101:1163:1000 2:N:0:TATCACTCTG+AACGTTACAT Sample_511 @A00132:144:HK735DMXX:1:1101:1868:1000 2:N:0:CTGAATTAGT+NATTCGATCG Sample_511 @A00404:186:HN3M2DMXX:1:1101:1488:1000 1:N:0:CTGAATTAGT+AATTCGATCG