I was looking at syn8612203 data and was wondering if the truseq protocol Mayo used was stranded or unstranded (most likely unstranded, but want to check).
Created by Panos Roussos panosr Yes, for the AMP-AD Mayo RNAseq the TruSeq RNA library prep kit was used, with is unstranded. The library prep kit I have in my notes (from way back...) for the Mayo RNAseq data is "TruSeq RNA Sample Prep Kit" ? which I'm pretty sure means it's unstranded.
I used the `U` flag (for "unstranded") when quantifying transcript abundance with Sailfish, so would also be interested in confirmation of this detail. :)
@mcarrasquillo - hi Minerva, I'm fairly sure this is un-stranded, but can you please confirm
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