Hello, I am reaching out to get some insight on the details of QC that was performed after the libraries were sequenced. I couldn't find anything regarding UMI cutoff, ambient RNA, mRNA expression, doublets and etc. Since the barcodes, features, and matrix files for the libraries could only map to some of the demultiplexing file barcodes, I am assuming that QC was done. I would greatly appreciate any information regarding the specific QC procedures, thresholds, and software used, as well as whether low-quality nuclei, doublets, or ambient RNA contamination were identified and addressed. Understanding the preprocessing steps would help me avoid repeating analyses that have already been performed. Thank you very much for any insight on this. Mykhailo Zaporozhets