Dear all, I was recently granted access to one of the ROSMAP studies, syn54847635 ("Dissecting the Human Leptomeninges at single-cell resolution" by Nicola A. Kearns et al.). For my project, I need to identify the NCI (non cognitive impairment) from bulk RNA seq FASTQ files (syn56426608) and distinguish them from the MCI and AD samples. To do this, I attempted to use the ROSMAP metadata files(syn3157322)—specifically the clinical metadata, codebook, and biospecimen metadata—to identify the control samples. However, I have encountered a dilemma: the authors of this study have used a different naming system for their files and samples than the original ROSMAP naming system. For the associated bulk RNA seq files, the samples are simply numbered from 1 to 44. For the FASTQ files, the naming follows a pattern of: Sample Name-Sample Index-Read Direction-001.fastq.gz. such as Menin-3-S76-R2-001.fastq.gz. In this example, Menin-3 appears to be a custom name assigned by the authors' team, rather than a name that corresponds to the original ROSMAP identifiers. I have contacted the authors personally but have not received a response. If anyone in this community could help me resolve this issue, I would be extremely grateful. I absolutely need to know which files belong to the NCI patients in order to conduct a scientifically sound analysis and experiment.

Created by Zahra Mirhendi z.mirhendi

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