I downloaded the hippocampus.rds (syn52408592) dataset for analysis, but I am having trouble identifying which samples correspond to controls and which correspond to patients. How can I determine the group identification, and in which variable are they stored in the .rds file? I want to integrate this snRNAseq dataset with my own lab dataset to identify shared biological mechanisms. Could you please explain how to approach this?
Data structure:
AD <- readRDS("Hippocampus.rds")
> head(AD)
projid cell_type_high_resolution major_cell_type
Thank you.
Created by Hasib Riaz hriaz Hello,
I can't find any explicit documentation but those look like sample number suffixes added by CellRanger. It looks like there is a 1:1 relationship between those and `projid` so I don't think they have any meaning beyond that. @jaclynbeck thank you so much. Can you please also explain or check meaning of these suffix added to the barcodes.
> head(barcodes)
[1] "AAAGGATAGTCCTACA-40-2" "AAAGGATAGTTTCGGT-40-2" "AAAGTCCGTACAAGCG-40-2" "AACAAAGTCCCAGCGA-40-2"
[5] "AACTTCTTCCCTAGGG-40-2" "AACTTCTTCCGAGCTG-40-2"
> unique(suffix)
[1] "40-2" "45-2" "54-2" "70-2" "75-2" "76-2" "77-2" "33-2" "30-2" "59-2" "35-2" "65-2" "25-2" "71-2" "22-2"
[16] "29-2" "66-2" "16-2" "69-2" "74-2" "81-2" "56-2" "58-2" "86-2" "64-2" "41-2" "73-2" "26-2" "27-2" "28-2"
[31] "78-2" "55-2" "63-2" "68-2" "46-2" "42-2" "72-2" "31-2" "43-2" "79-2" "34-2" "39-2" "48-2" "61-2" "36-2"
[46] "67-2" "37-2" "17-2".
Thank you. Hello,
I'm so sorry for the delayed response. I took a look at the file, and it looks like in the Seurat metadata there's a field called `projid`. You can use this as the identifier to match the `projid` column in the [ROSMAP metadata file ](https://www.synapse.org/Synapse:syn3191087), which will have the diagnosis data.
I hope that helps!
Jaclyn Beck I am able to get AAAGGATAGTCCTACA-40-2 labelling (e.g "16-2" "17-2" "22-2" "25-2"), Where i can get their control and disease condition identification. Please help to figure-out this.
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