I am working on snRNAseq DLPFC #2 data set. I am interested in only AD and control individuals (dcfdc_lv= 1 OR 4), so I only downloaded those count matrices. I downloaded 123 count matrices (all of them except 191122-B6-R1710143-alone", "191122-B6-R1969233-alone" ,"191122-B6-R4078277-alone","191122-B6-R6622577-alone" which don't have dcfdc_lv= 1 OR 4 samples). I am trying to demultiplex. My merged SEURAT object has only 6698007 samples while the ROSMAP_snRNAseq_demultiplexed_ID_mapping.csv file (filtered for dcfdc_lv= 1 OR 4 individuals) has 12998832 samples. This discrepancy does not make sense. Please help! I created seurat objects first, then merged.

Created by Ashay Patel aopatel
Hello any reply for my concern would be much appreciated. This is preventing me from moving on with my analysis. Once again the count matrix samples, from my end, are far too low compared with the samples in the demultiplex file (which I already filtered for individuals of interest for my investigation). Thank you for your time,

Discrepency between count matrix and demultiedID file? page is loading…