Dear AMPAD, We have recently tried to process the raw RNA-seq files from the ROSMAP_MammillaryBody study (study: syn50670858, files: syn50670875). However, we found that some files in this dataset seem to be broken. Specifically, some files cannot be fully ungzipped (they give a CRC error), and other files seem to have fewer lines than their matching read pair / lane counterpart. We suspect that these files might have become currupted while they were uploaded to the Synapse repository: we redownloaded some of these files, but also the newly downloaded copies have error, while the filesizes match those listed on the website / metadata. A list of files with issues and their problems is below. Could you verify whether this is the case? Hopefully this will help you fix the repository. With kind regards, Harm-Jan Westra, UMCG **File name Error** HL2CNDMXX_D19-7253_S6_L001_R2_001.fastq.gz gzip: invalid compressed data--crc error; invalid compressed data--length error HL2CNDMXX_D19-7266 _S19_L001_R2_001.fastq.gz gzip: invalid compressed data--crc error; invalid compressed data--length error HL2CNDMXX_D19-7266 _S19_L002_R2_001.fastq.gz gzip: invalid compressed data--crc error; invalid compressed data--length error HL2CNDMXX_D19-7267_S20_L002_R1_001.fastq.gz gzip: invalid compressed data--crc error; invalid compressed data--length error HL2CNDMXX_D19-7267_S20_L002_R2_001.fastq.gz gzip: invalid compressed data--crc error; invalid compressed data--length error HL2CNDMXX_D19-7268_S21_L001_R1_001.fastq.gz gzip: invalid compressed data--crc error; invalid compressed data--length error HL2CNDMXX_D19-7268_S21_L001_R2_001.fastq.gz gzip: invalid compressed data--crc error; invalid compressed data--length error HL2CNDMXX_D19-7268_S21_L002_I1_001.fastq.gz gzip: invalid compressed data--crc error; invalid compressed data--length error HL73LDMXX_D19-7248_S1_L002_R1_001.fastq.gz Number of lines does not match L002_I1_001 and L002_R2_001 HL73LDMXX_D19-7249_S2_L002_R1_001.fastq.gz Number of lines does not match L002_I1_001 and L002_R2_001 HL73LDMXX_D19-7250_S3_L002_R1_001.fastq.gz Number of lines does not match L002_I1_001 and L002_R2_001 HL73LDMXX_D19-7251_S4_L002_R1_001.fastq.gz Number of lines does not match L002_I1_001 and L002_R2_001 HL73LDMXX_D19-7252_S5_L002_R1_001.fastq.gz Number of lines does not match L002_I1_001 and L002_R2_001 HL73LDMXX_D19-7253_S6_L002_R1_001.fastq.gz Number of lines does not match L002_I1_001 and L002_R2_001 HL73LDMXX_D19-7254_S7_L002_R1_001.fastq.gz Number of lines does not match L002_I1_001 and L002_R2_001 HL73LDMXX_D19-7255_S8_L002_R1_001.fastq.gz Number of lines does not match L002_I1_001 and L002_R2_001 HL73LDMXX_D19-7256_S9_L002_R1_001.fastq.gz Number of lines does not match L002_I1_001 and L002_R2_001 HL73LDMXX_D19-7257_S10_L002_R1_001.fastq.gz Number of lines does not match L002_I1_001 and L002_R2_001 HL73LDMXX_D19-7258_S11_L002_R1_001.fastq.gz Number of lines does not match L002_I1_001 and L002_R2_001 HL73LDMXX_D19-7259_S12_L002_R1_001.fastq.gz Number of lines does not match L002_I1_001 and L002_R2_001 HL73LDMXX_D19-7260_S13_L002_R1_001.fastq.gz Number of lines does not match L002_I1_001 and L002_R2_001 HL73LDMXX_D19-7261_S14_L002_R1_001.fastq.gz Number of lines does not match L002_I1_001 and L002_R2_001 HL73LDMXX_D19-7262_S15_L002_R1_001.fastq.gz Number of lines does not match L002_I1_001 and L002_R2_001 HL73LDMXX_D19-726_S16_L002_R1_001.fastq.gz Number of lines does not match L002_I1_001 and L002_R2_001 HL73LDMXX_D19-7264_S17_L002_R1_001.fastq.gz Number of lines does not match L002_I1_001 and L002_R2_001 HL73LDMXX_D19-7265_S18_L002_R1_001.fastq.gz Number of lines does not match L002_I1_001 and L002_R2_001 HL73LDMXX_D19-7266_S19_L002_R1_001.fastq.gz Number of lines does not match L002_I1_001 and L002_R2_001 HL73LDMXX_D19-7267_S20_L002_R1_001.fastq.gz Number of lines does not match L002_I1_001 and L002_R2_001 HL73LDMXX_D19-7268_S21_L002_R1_001.fastq.gz Number of lines does not match L002_I1_001 and L002_R2_001 HL73LDMXX_D19-7269_S22_L002_R1_001.fastq.gz Number of lines does not match L002_I1_001 and L002_R2_001 HL73LDMXX_D19-7270_S23_L002_R1_001.fastq.gz Number of lines does not match L002_I1_001 and L002_R2_001 HL73LDMXX_D19-7271_24_L002_R1_001.fastq.gz Number of lines does not match L002_I1_001 and L002_R2_001 HL73LDMXX_D19-7272_25_L002_R1_001.fastq.gz Number of lines does not match L002_I1_001 and L002_R2_001 HL73LDMXX_D19-7273_26_L002_R1_001.fastq.gz Number of lines does not match L002_I1_001 and L002_R2_001 HL73LDMXX_D19-7274_27_L002_R1_001.fastq.gz Number of lines does not match L002_I1_001 and L002_R2_001 HL73LDMXX_D19-7275_28_L002_R1_001.fastq.gz Number of lines does not match L002_I1_001 and L002_R2_001 HL73LDMXX_D19-7276_29_L002_R1_001.fastq.gz Number of lines does not match L002_I1_001 and L002_R2_001 HL73LDMXX_D19-7277_30_L002_R1_001.fastq.gz Number of lines does not match L002_I1_001 and L002_R2_001 HL73LDMXX_D19-7278_31_L002_R1_001.fastq.gz Number of lines does not match L002_I1_001 and L002_R2_001 HL73LDMXX_D19-7279_32_L002_R1_001.fastq.gz Number of lines does not match L002_I1_001 and L002_R2_001